Journal: Scientific reports

Article Title: Suppressive action of nesfatin-1 and nesfatin-1-like peptide on cortisol synthesis in human adrenal cortex cells.

doi: 10.1038/s41598-024-54758-7

Figure Lengend Snippet: Figure 3. NLP and NESF-1 decreased the phosphorylation of CREB in H295R cells (A) and regulated cortisol release through a CREB mediated pathway (B). The results presented are pooled from 3 independent studies with duplicate/triplicate for each treatment. Same alphabets show no significant difference between groups, while different letters indicate significant differences among groups. Full size western gel images that served as the source for this figure are shown in Supplementary Fig. 2. Each of the image came from a single source gel image.

Article Snippet: Primary antibodies used in this study were monoclonal mouse anti-beta-actin antibody [1:1000, RRID: AB_528068, cat no. JLA20, Developmental Studies Hybridoma Bank, DSHB, University of Iowa, USA], polyclonal rabbit anti-phospho [Ser 133]-CREB antibody [1:1000, RRID: AB_2561044, cat no. 9198, Cell Signalling, USA], 11 Vol.

Techniques: Phospho-proteomics, Western Blot